pcdna3 1 klotho (Addgene inc)
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pcdna3 1 klotho
Pcdna3 1 Klotho, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+klotho/Klotho+(membrane)+(Plasmid+%2317712)/pmc06698229-441-15-16
Average 93 stars, based on 20 article reviews
Pcdna3 1 Klotho, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+klotho/Klotho+(membrane)+(Plasmid+%2317712)/pmc06698229-441-15-16
Average 93 stars, based on 20 article reviews
pcdna3 1 klotho - by Bioz Stars,
2026-09
93/100 stars
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Knockdown:Article Title: Extracellular Vesicles from Albumin-Induced Tubular Epithelial Cells Promote the M1 Macrophage Phenotype by Targeting Klotho Article Snippet: Cell Culture and Treatment A human proximal tubule cell line (HK-2) and a human monocyte cell line (THP-1) purchased from the Cell Bank of the Type Culture Collection (Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium (Invitrogen, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; GIBCO, Australia); before treatment, cells were cultured in 2% FBS RPMI 1640 medium for 24 h. THP-1 cells were differentiated into macrophage cells through 100 nM PMA (S1819; Beyotime, China) induction for 48 h. For miR-199a-5p overexpression or inhibition, 50 nM miR-199a-5p mimic or 100 nM inhibitor (RiboBio, Guangzhou, China), respectively, was transfected into macrophage cells or HK-2 cells. .. For Klotho knockdown or overexpression, 50 nM si-Klotho (RiboBio, Guangzhou, China) or 100 ng of Over Expression:Article Title: Extracellular Vesicles from Albumin-Induced Tubular Epithelial Cells Promote the M1 Macrophage Phenotype by Targeting Klotho Article Snippet: Cell Culture and Treatment A human proximal tubule cell line (HK-2) and a human monocyte cell line (THP-1) purchased from the Cell Bank of the Type Culture Collection (Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium (Invitrogen, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; GIBCO, Australia); before treatment, cells were cultured in 2% FBS RPMI 1640 medium for 24 h. THP-1 cells were differentiated into macrophage cells through 100 nM PMA (S1819; Beyotime, China) induction for 48 h. For miR-199a-5p overexpression or inhibition, 50 nM miR-199a-5p mimic or 100 nM inhibitor (RiboBio, Guangzhou, China), respectively, was transfected into macrophage cells or HK-2 cells. .. For Klotho knockdown or overexpression, 50 nM si-Klotho (RiboBio, Guangzhou, China) or 100 ng of Plasmid Preparation:Article Title: Extracellular Vesicles from Albumin-Induced Tubular Epithelial Cells Promote the M1 Macrophage Phenotype by Targeting Klotho Article Snippet: Cell Culture and Treatment A human proximal tubule cell line (HK-2) and a human monocyte cell line (THP-1) purchased from the Cell Bank of the Type Culture Collection (Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium (Invitrogen, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; GIBCO, Australia); before treatment, cells were cultured in 2% FBS RPMI 1640 medium for 24 h. THP-1 cells were differentiated into macrophage cells through 100 nM PMA (S1819; Beyotime, China) induction for 48 h. For miR-199a-5p overexpression or inhibition, 50 nM miR-199a-5p mimic or 100 nM inhibitor (RiboBio, Guangzhou, China), respectively, was transfected into macrophage cells or HK-2 cells. .. For Klotho knockdown or overexpression, 50 nM si-Klotho (RiboBio, Guangzhou, China) or 100 ng of Transfection:Article Title: Extracellular Vesicles from Albumin-Induced Tubular Epithelial Cells Promote the M1 Macrophage Phenotype by Targeting Klotho Article Snippet: Cell Culture and Treatment A human proximal tubule cell line (HK-2) and a human monocyte cell line (THP-1) purchased from the Cell Bank of the Type Culture Collection (Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium (Invitrogen, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; GIBCO, Australia); before treatment, cells were cultured in 2% FBS RPMI 1640 medium for 24 h. THP-1 cells were differentiated into macrophage cells through 100 nM PMA (S1819; Beyotime, China) induction for 48 h. For miR-199a-5p overexpression or inhibition, 50 nM miR-199a-5p mimic or 100 nM inhibitor (RiboBio, Guangzhou, China), respectively, was transfected into macrophage cells or HK-2 cells. .. For Klotho knockdown or overexpression, 50 nM si-Klotho (RiboBio, Guangzhou, China) or 100 ng of |